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Dendritic Cells: Biophysics, Tumor Microenvironment and by Zhu Zeng, Xiaofeng Xu, Dan Chen

By Zhu Zeng, Xiaofeng Xu, Dan Chen

In this e-book, the authors talk about the biophysical features of Dendritic cells (DCs) at quite a few differentiation levels and in tumor microenvironments intimately. one of those chinese language conventional herb, that may increase the biophysical and microrheological homes of DCs, is additionally brought. DCs are powerful and really good antigen-presenting cells that play a very important function in starting up and amplifying either the innate and adaptive immune responses. The biophysical and microrheological features of DCs are vital for his or her motility and basic immune functionality. In tumor microenvironments, the motility and immune capabilities of DCs are impaired, however the mechanisms are principally unknown. This publication bargains researchers a complete and deep perception into the biophysical features of DCs and should additionally aid to enhance the potency of anti-tumor treatment in line with DCs.

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The data represent mean ± SD. 05, receptively). 1 0 DCs DCs+nonGF DCs+HCC Fig. 4 The fluorescence polarization parameter (p) of DCs. DCs cultured with and without growth factor (GF) and co-cultured with HCCs are labeled as DCs, DCs + nonGF, and DCs + HCC, respectively. 05 (cited from Cell Biochemistry and Biophysics, 2009, 55(1):33–43) 4 Biophysical Characteristics of DCs in Tumor Microenvironment 31 The percentage of non-hemolyzed cells (%) (a) 100 90 * 80 70 * 60 ** imDCs 50 mDCs 40 ** imDCs+SJC 30 mDCs+SJC 20 10 0 0 25 50 75 100 125 150 175 200 225 250 275 300 Osmotic pressure (mOsm/kg) Percentagfe of hemolyzhed cells (%) (b) imDC+nonGF 100 imDC 90 mDC 80 * imDC+HCC 70 ** 60 mDC+HCC 50 40 ** 30 ** ** 20 10 0 0 25 50 75 100 125 150 175 200 225 250 275 300 Osmotic pressure (mOsm/kg) Fig.

2 kDa). The numbers of cells that had endocytosed the particles were determined by flow cytometry. The data represent mean ± SD. These measurements were performed in triplicates under the same condition. a SJC (cited from Cell Motility and Cytoskeleton, 2007, 64(3):186–198); b HCC (cited from Cell Biochemistry and Biophysics, 2009, 55(1):33–43); c K562 (cited from Journal of Biomechanics, 2010, 43(12):2339–2347). 01 Vibrational spectroscopy offers complete information on the chemical composition of samples, regarding both major and minor compounds, which presents many characteristic bands in the infrared range (IR).

01 (cited from Biomedical Engineering Online. 1) and apoptosis analyses (Fig. 17) showed that the TMDF did not affect the viability and apoptosis of DCs, suggesting that the TMDF caused the impairment of biophysical characteristics of DCs rather than cell death. To explore the potential molecular mechanisms of above-mentioned data, the microarray analysis and two-dimensional electrophoresis-based proteomics were performed. 05) compared with untreated mDCs. Gene ontology analysis showed that these genes are associated with cellular functions such as immunology, metabolism, cell migration, apoptosis, etc.

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